| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139417 |
| Chromosome: | chromosome 4 |
| Location: | 2945731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224700 | NRT1 | (1 of 1) K14638 - solute carrier family 15 (peptide/histidine transporter), member 3/4 (SLC15A3_4, PHT); Nitrate transporter NRT1 family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACACGGGGATGAGTAGAATGATGGAGAG |
| Internal bar code: | CAACCACGTCTGTCAATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 99.7044 |
| LEAP-Seq n confirming: | 3036 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAAGTAAACCAAGCCAA |
| Suggested primer 2: | GGAGGGGAACCATTTCATTT |