| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139419 |
| Chromosome: | chromosome 3 |
| Location: | 6296892 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193150 | (1 of 1) PTHR13507//PTHR13507:SF0 - UNCHARACTERIZED // PRKR-INTERACTING PROTEIN 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTGACTTGAGGCAACACAGTAGTAAT |
| Internal bar code: | CAGACACCGGGAATTCGTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 99.5677 |
| LEAP-Seq n confirming: | 10826 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTACCGTACTGGAACC |
| Suggested primer 2: | CCTCCTCGTTTGACTTCTGC |