| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139429 |
| Chromosome: | chromosome_6 |
| Location: | 1206799 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257650 | MSRA2 | Peptide methionine-S-sulfoxide reductase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTTTGTACGGCAGGGGGTCTCGTCGGTG |
| Internal bar code: | ACCGGGGGCCGTCGCGGCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 23 |
| LEAP-Seq percent confirming: | 99.7821 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTCAAGAGGAGGTGTCG |
| Suggested primer 2: | ACCTCTGCGTGTCCTGTCTT |