| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139433 |
| Chromosome: | chromosome 17 |
| Location: | 2501326 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716101 | POLL1 | (1 of 1) PF10391 - Fingers domain of DNA polymerase lambda (DNA_pol_lambd_f); DNA polymerase lambda | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGCGAGGGCCGAGGTGGCGGAGGCG |
| Internal bar code: | GTTGCCCTCTTCGCCACGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 358 |
| LEAP-Seq percent confirming: | 99.6403 |
| LEAP-Seq n confirming: | 1385 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATGGAAGGTGGCAGGT |
| Suggested primer 2: | TCGTGTGTTTGGTTTCGTGT |