Insertion junction: LMJ.RY0402.139442_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.139442
Chromosome:chromosome 5
Location:1840460
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g241950 VDAC2,ASC2 (1 of 2) K15040 - voltage-dependent anion channel protein 2 (VDAC2); Voltage-dependent anion-selective channel protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGAAGTAGCTCGTGGACGGGCTCGCCT
Internal bar code:TATCACATTCAGTGATTTAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:246
LEAP-Seq percent confirming:98.4663
LEAP-Seq n confirming:321
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGACCGTAAACCAGACACCG
Suggested primer 2:TGTACATGTGTGTGCGTTGG