| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139443 |
| Chromosome: | chromosome 6 |
| Location: | 3023051 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273850 | HTB7 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTAAAAGCTCTCAGATGCTGGACCGTG |
| Internal bar code: | TCGAGTTTACTGGTAGTACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 99.6264 |
| LEAP-Seq n confirming: | 7733 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTTTCCAGTTTGCCCAC |
| Suggested primer 2: | TCTTCAGGTAGCGTGCAATG |