| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139464 |
| Chromosome: | chromosome 12 |
| Location: | 1756945 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512450 | (1 of 12) PF04749 - PLAC8 family (PLAC8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCGAATCTGTGATTACTGCACTCGTCG |
| Internal bar code: | CCGTATAGGTTCACCCATTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.5279 |
| LEAP-Seq n confirming: | 5060 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTTACATGTCGGGTGGC |
| Suggested primer 2: | GCATGTAGAGGTAGAGCGGC |