| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139472 |
| Chromosome: | chromosome 12 |
| Location: | 4847359 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524950 | ENGB,ENGB1 | Factor possbly involved in ribosomal 50S subunit assembly; (1 of 1) PTHR11649//PTHR11649:SF49 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCGGTGATCGGCCGCAGCAATGTGGG |
| Internal bar code: | TTTTGCGCGCCTAGACTAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 98.4709 |
| LEAP-Seq n confirming: | 22668 |
| LEAP-Seq n nonconfirming: | 352 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGAATGATGATGAGGAC |
| Suggested primer 2: | GCACGAGGAGATCGTATGGT |