| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139484 |
| Chromosome: | chromosome 2 |
| Location: | 6027034 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112500 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTGGGAGCGGGTGTCGTCAGACGATGA |
| Internal bar code: | TACGCGACGGTATAGTCCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 99.7881 |
| LEAP-Seq n confirming: | 1413 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAGGCCTCGGCTAAGTG |
| Suggested primer 2: | CTAGATTGCGAGGTGTGCAA |