| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139485 |
| Chromosome: | chromosome 15 |
| Location: | 1338292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641950 | (1 of 1) PF14846 - Domain of unknown function (DUF4485) (DUF4485) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGAACGGGCACCAGGGGAGTGGTGGGG |
| Internal bar code: | CCGTACTACCGCGAGGCCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 99.737 |
| LEAP-Seq n confirming: | 2275 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGGTGCTTAGGTGGAG |
| Suggested primer 2: | CTGTTGAAACGGAGGCTGTT |