Insertion junction: LMJ.RY0402.139508_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139508
Chromosome:chromosome 6
Location:7362093
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g299150 PHC9,PHC26 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 26 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATATTCCCGTGTTGACGACAACTAATCC
Internal bar code:CGGGAGTGCGATATAGGGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:860
LEAP-Seq percent confirming:99.6084
LEAP-Seq n confirming:8902
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTCCACAGAAAAAGCAG
Suggested primer 2:CATACGTACATACGCGGTGC