| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139512 |
| Chromosome: | chromosome 13 |
| Location: | 1476590 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572401 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGGAGAGCGCGGCGACATTTGCAGGA |
| Internal bar code: | GCGAGGAGAGAGTAGAGTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 99.6168 |
| LEAP-Seq n confirming: | 9359 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGTTGGACAAAGAAAA |
| Suggested primer 2: | TGCATCAACCGACAAACAAT |