| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139531 |
| Chromosome: | chromosome 7 |
| Location: | 1487686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323750 | HAS2 | (1 of 1) K06133 - 4'-phosphopantetheinyl transferase (LYS5, acpT); Holo-[acyl-carrier-protein] synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATGGGTCTCTTCTACTCGTTTCATTA |
| Internal bar code: | TGAGTTGGTGGGCGGGAGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.8322 |
| LEAP-Seq n confirming: | 4759 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGAAGTGGGGGCCTAT |
| Suggested primer 2: | CAACTTGACCCCCAGAAAGA |