| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139546 |
| Chromosome: | chromosome 11 |
| Location: | 2966541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478184 | (1 of 1) 3.2.1.25 - Beta-mannosidase / Mannase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTATCTAAGAAGGGGAGGGGTGTTGTGC |
| Internal bar code: | AGTCATTAAAGGGGTTGAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 92.7787 |
| LEAP-Seq n confirming: | 1606 |
| LEAP-Seq n nonconfirming: | 125 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATAACCCTTCCCTTGTGC |
| Suggested primer 2: | AGCGGTATAAAGGCAGCAGA |