| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139554 |
| Chromosome: | chromosome 13 |
| Location: | 5189602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607900 | CPLD68 | Conserved in the Plant Lineage and Diatoms; (1 of 96) IPR019734 - Tetratricopeptide repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCCTTCTGGGCTTTCCTTCCTATTACA |
| Internal bar code: | TCCAGGCCCTCTTAGGCGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1242 |
| LEAP-Seq percent confirming: | 99.7256 |
| LEAP-Seq n confirming: | 7995 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATGTGACTCATTGCAGC |
| Suggested primer 2: | GGAGGACGTATACAAGCCCA |