| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139582 |
| Chromosome: | chromosome_8 |
| Location: | 2396167 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g371000 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGACGGGCGTCAGGGCCGGACTGAGGGGC |
| Internal bar code: | CGCACGCGAATTCATCTGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 84.3863 |
| LEAP-Seq n confirming: | 2097 |
| LEAP-Seq n nonconfirming: | 388 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCCTATGGTCCTGTGTT |
| Suggested primer 2: | TAGAAGCCGACAAGCAGGTT |