| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139584 |
| Chromosome: | chromosome 8 |
| Location: | 1076928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362200 | (1 of 226) IPR012337 - Ribonuclease H-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTCAATCGATGGTTACGTTGGTACGGG |
| Internal bar code: | GTGGAATAGTCAATGCGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 98.666 |
| LEAP-Seq n confirming: | 7470 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACTGCTAGATAGGCACC |
| Suggested primer 2: | TAGTGCTTCTACTTCGCGGC |