| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139588 |
| Chromosome: | chromosome 7 |
| Location: | 2620043 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAGAGGACCGGTCAGTCTTTGCGGGGG |
| Internal bar code: | ATACCCCCGGGATTCGCAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 99.097 |
| LEAP-Seq n confirming: | 5048 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCAATGGTCTGAACTGC |
| Suggested primer 2: | CTGGGAGAGTTCGAGACTGG |