| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139634 |
| Chromosome: | chromosome 16 |
| Location: | 5640103 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679450 | (1 of 1) K14566 - U3 small nucleolar RNA-associated protein 24 (UTP24, FCF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAGGGTTTTGGCTGTGGCCAGCGTGCT |
| Internal bar code: | AATCGTGAAGGGAACGGGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.4475 |
| LEAP-Seq n confirming: | 1080 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACGACACAAACCAGAC |
| Suggested primer 2: | AGCATTTCCGTCTGTGCTCT |