Insertion junction: LMJ.RY0402.139634_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.139634
Chromosome:chromosome 16
Location:5640108
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g679450 (1 of 1) K14566 - U3 small nucleolar RNA-associated protein 24 (UTP24, FCF1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTTGGGCATGCTCTCACACATCATGCTG
Internal bar code:ACGCGTCTACGTCACTACAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:139
LEAP-Seq percent confirming:81.4697
LEAP-Seq n confirming:255
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACCACGACACAAACCAGAC
Suggested primer 2:AGCATTTCCGTCTGTGCTCT