| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139640 |
| Chromosome: | chromosome 12 |
| Location: | 7278438 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557800 | (1 of 1) K06875 - programmed cell death protein 5 (PDCD5, TFAR19) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGGGCCGGCGCCGCTGTATCGTGATC |
| Internal bar code: | TCACGTGAAGACGGGCACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 98.1444 |
| LEAP-Seq n confirming: | 4866 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGCTCAGTACTTGGTCC |
| Suggested primer 2: | GTGATACCCGTGTGCTCCTT |