| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139666 |
| Chromosome: | chromosome 1 |
| Location: | 2299264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012600 | GPM2,PGM2 | Phosphoglucomutase 2; (1 of 1) 5.4.2.7 - Phosphopentomutase / Phosphodeoxyribomutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACAAACCCCCGACAGCCCTCGCTTCAGC |
| Internal bar code: | TGGCGTAGGCATCAGAGCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.4257 |
| LEAP-Seq n confirming: | 1385 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACTAGACGGTGCGCTTG |
| Suggested primer 2: | GGCCTGACACGTTAGACCAT |