| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139680 |
| Chromosome: | chromosome 16 |
| Location: | 7573353 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688863 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCGCCCGGGCCAGACCGCATCAACACC |
| Internal bar code: | GGGAGGGGGCCGTCCGGAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 88.5186 |
| LEAP-Seq n confirming: | 30708 |
| LEAP-Seq n nonconfirming: | 3983 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTGGCAGTCATACAGG |
| Suggested primer 2: | TTTCCTTGTGGGACTGGTTC |