| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139688 |
| Chromosome: | chromosome 16 |
| Location: | 6958372 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677093 | (1 of 10) PTHR12270//PTHR12270:SF6 - GLYCOSYLTRANSFERASE-RELATED // GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAATGCTGCTGGAAGGCACTGCTGCAA |
| Internal bar code: | GTGGGAGTCCAGTATGGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 98.45 |
| LEAP-Seq n confirming: | 7368 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGAACTGATTCACAGCA |
| Suggested primer 2: | AGCGCTGTTGGAGGAATAGA |