| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139688 |
| Chromosome: | chromosome 16 |
| Location: | 7196017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681802 | (1 of 2) PF00069//PF01590//PF07714 - Protein kinase domain (Pkinase) // GAF domain (GAF) // Protein tyrosine kinase (Pkinase_Tyr) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCTTCGAGGTGAGGTCGCCCGCGCGG |
| Internal bar code: | CCTGCCATAAAAAAGGTACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 96.6109 |
| LEAP-Seq n confirming: | 30844 |
| LEAP-Seq n nonconfirming: | 1082 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTGAACCCACTTTCACC |
| Suggested primer 2: | CCAGCTCTTACCAGCTTTGG |