| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139693 |
| Chromosome: | chromosome_2 |
| Location: | 6517588 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g116650 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTAATTGAAGCTGTGCACGACGCATCTAG |
| Internal bar code: | TTTGGGCTTCGTTATCAGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.1093 |
| LEAP-Seq n confirming: | 3227 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTAGCCGGAAGACCACAT |
| Suggested primer 2: | TCGCCACACACACGTTTTAT |