| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139700 |
| Chromosome: | chromosome 13 |
| Location: | 1810505 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575101 | (1 of 726) IPR011009 - Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACGCGCTGCCAGCTCTTACATTCCGT |
| Internal bar code: | TAGGAGCAGTAGTGTTCATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.2401 |
| LEAP-Seq n confirming: | 8358 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAAAGATACTCCTGCAT |
| Suggested primer 2: | TTACACGGTTGACACTGGGA |