| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139703 |
| Chromosome: | chromosome 6 |
| Location: | 6967433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296400 | (1 of 1) 1.3.8.4 - Isovaleryl-CoA dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCACGCGTACGCACAAACGTGTACAG |
| Internal bar code: | GCCCGTTAGGCTGACAGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 97.7927 |
| LEAP-Seq n confirming: | 41336 |
| LEAP-Seq n nonconfirming: | 933 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTTCTTGCTTTCCGTG |
| Suggested primer 2: | TGACATGCAGACAACAAGCA |