Insertion junction: LMJ.RY0402.139708_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139708
Chromosome:chromosome 1
Location:5419906
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g038050 COP7,HKR3,COP Histidine kinase rhodopsin 3; (1 of 1) PF00072//PF00512//PF01036 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGGTGCTTGGTGGTTCAGCCAGTTGGC
Internal bar code:GAACGACCCGGGCGCCACGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:759
LEAP-Seq percent confirming:94.4534
LEAP-Seq n confirming:11103
LEAP-Seq n nonconfirming:652
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTCAAAATAACGCGCAC
Suggested primer 2:TACCCTACCTGCCTCACACC