| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139729 |
| Chromosome: | chromosome 7 |
| Location: | 2571998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329882 | VGK1,VGKC | Ion transport protein; (1 of 1) PTHR10217//PTHR10217:SF503 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGAGCTATGGTGCCTCTGGGTGCCTGG |
| Internal bar code: | GCTGTTCATGGATAATGACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.4357 |
| LEAP-Seq n confirming: | 10573 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTGTCCAGGGACACCAC |
| Suggested primer 2: | GAACGTGTCACTGGTGATGG |