| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139732 |
| Chromosome: | chromosome 2 |
| Location: | 7123670 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146650 | TTL3,SSA11 | (1 of 2) K16582 - tubulin polyglutamylase TTLL6/13 [EC:6.-.-.-] (TTLL6_13); Tubulin tyrosine ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTCTCGCTGCAGCTGTACTGGACGGA |
| Internal bar code: | TGCTGTCGAATTCGATGACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGATGGTGCTAGGAAAGC |
| Suggested primer 2: | GTTCATGGGTGGCAGCTTAT |