| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139749 |
| Chromosome: | chromosome 10 |
| Location: | 1034168 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425100 | (1 of 1) PTHR24185:SF1 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCCCTGGGGGGGGGCCAGGAGAGGTG |
| Internal bar code: | CCCCGTGTCAGCGGCATCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.7213 |
| LEAP-Seq n confirming: | 1431 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAATTTTGTTGGGGTTCA |
| Suggested primer 2: | GAAAACATTGCGCAAAGACA |