| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139752 |
| Chromosome: | chromosome 3 |
| Location: | 1804430 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154300 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAAGAGTCTCAACCATGGCCCCGAAGAA |
| Internal bar code: | CGATGCCCGCAGGCGGTGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 41.0528 |
| LEAP-Seq n confirming: | 4687 |
| LEAP-Seq n nonconfirming: | 6730 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGGAGAGGTTGTGGGTG |
| Suggested primer 2: | GAGGCAGCTGTTGGCTCTAC |