| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139756 |
| Chromosome: | chromosome 9 |
| Location: | 3965750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392579 | MRPL115,OPR10,mL115 | (1 of 239) IPR016024 - Armadillo-type fold; mitochondrial ribosome protein mL115, Chlamydomonas-specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGAGGCTACATCATACCGGTAACCTC |
| Internal bar code: | CGGTGTGGGGTTAGCGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 94.2518 |
| LEAP-Seq n confirming: | 4132 |
| LEAP-Seq n nonconfirming: | 252 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGTATGCTGTATGGCTG |
| Suggested primer 2: | CTGCTGCAGACCCTCCTTAG |