Insertion junction: LMJ.RY0402.139757_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.139757
Chromosome:chromosome 1
Location:5226151
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g036350 PMA1 (1 of 5) K01537 - Ca2+-transporting ATPase (E3.6.3.8); Related to plasma membrane P-type and chloroplast type calcium ATPases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGATGGTGGGCAACCGGACCGAGTGCGC
Internal bar code:CCATACCAACTTCGAGTGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:454
LEAP-Seq percent confirming:7.89553
LEAP-Seq n confirming:783
LEAP-Seq n nonconfirming:9134
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCACCAGCAGTAGCCATA
Suggested primer 2:TAAGGTGTGTTTCGGGAAGG