| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139774 |
| Chromosome: | chromosome 6 |
| Location: | 4266714 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278249 | AST5 | Putative aspartate aminotransferase; (1 of 1) PTHR11751//PTHR11751:SF33 - SUBGROUP I AMINOTRANSFERASE RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCTGGACCGCACCACCCCAACACCCCA |
| Internal bar code: | GTTGCGAGCGAGGAAATTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 37 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGTCGTCGTGTCCCTAC |
| Suggested primer 2: | TATGTTATGCAGCCTCTGCG |