| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139783 |
| Chromosome: | chromosome 1 |
| Location: | 2331534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012750 | KCN7 | (1 of 4) IPR000595//IPR002110//IPR005821//IPR018490//IPR020683 - Cyclic nucleotide-binding domain // Ankyrin repeat // Ion transport domain // Cyclic nucleotide-binding-like // Ankyrin repeat-containing domain; Voltage-gated potassium channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTCAACTTCGACTCCGCACACGCCGGC |
| Internal bar code: | TCCACGCGGGTGGTTCAGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 99.7734 |
| LEAP-Seq n confirming: | 25101 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAATACACCACTCACCG |
| Suggested primer 2: | ATGATGCTCACTCCTCGCTT |