Insertion junction: LMJ.RY0402.139815_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139815
Chromosome:chromosome 2
Location:3313584
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095087 (1 of 2) 3.4.14.1 - Dipeptidyl-peptidase I / Dipeptidyl transferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTGTACATGCGATTAGGCGTCTCACAC
Internal bar code:CGACCCTAGGGTGGCGCCGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:918
LEAP-Seq percent confirming:99.5521
LEAP-Seq n confirming:24447
LEAP-Seq n nonconfirming:110
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTATGTCCCATGCGTAG
Suggested primer 2:GCCACAACTAAAAGTCCCCA