Insertion junction: LMJ.RY0402.139821_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.139821
Chromosome:chromosome 16
Location:1307475
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651600 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGTTGCATTGTCCCTTCTTGTAACCTT
Internal bar code:ATCAAAGGGAGCGCATAATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:98.0299
LEAP-Seq n confirming:6618
LEAP-Seq n nonconfirming:133
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:ATGATTTTCGCTTGGCATTC
Suggested primer 2:GTCAGGCTGCTAACGGTTTC