| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139822 |
| Chromosome: | chromosome_3 |
| Location: | 4497403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g176300 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCATCCAAGCCACAGACACAGCCCCCGAC |
| Internal bar code: | GCCACAACCGCCTCGGGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 46.8266 |
| LEAP-Seq n confirming: | 605 |
| LEAP-Seq n nonconfirming: | 687 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGTCCCTTCATTTCTCA |
| Suggested primer 2: | TGCTGGTCGATTCTTCTGTG |