Insertion junction: LMJ.RY0402.139826_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.139826
Chromosome:chromosome 1
Location:5456716
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCGGCGGGATTGAATATGGATGTAGGTC
Internal bar code:TATGCGTCGTCGGTCGGGGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:890
LEAP-Seq percent confirming:99.1015
LEAP-Seq n confirming:6949
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAGCAACAGGACTCGCTT
Suggested primer 2:CACTCGCAGCTTTGATGTGT