Insertion junction: LMJ.RY0402.139826_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.139826
Chromosome:chromosome 12
Location:7261073
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g558100 PRMT2,PRMT10,PRM2 Protein-/Histone-arginine N-methyltransferase; (1 of 1) PTHR11006:SF68 - PROTEIN ARGININE N-METHYLTRANSFERASE PRMT10 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGAGCAGAGCAAAACGCCGCGCGAGTTC
Internal bar code:CCCAGTATTCCCGTGTCACAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:971
LEAP-Seq percent confirming:98.8856
LEAP-Seq n confirming:18634
LEAP-Seq n nonconfirming:210
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCAGGCTAGCTAACCCAT
Suggested primer 2:ACGAGAACCCCACAGACAAC