| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139848 |
| Chromosome: | chromosome 12 |
| Location: | 1110630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCCCCTCGTCCCGCCAGTGTACAGGCT |
| Internal bar code: | GCCGGGCAATTACGGACTACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1246 |
| LEAP-Seq percent confirming: | 99.5674 |
| LEAP-Seq n confirming: | 12430 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCAGCTATGAGCGTGTG |
| Suggested primer 2: | ACATCTTGTCGGTGGAGACC |