Insertion junction: LMJ.RY0402.139868_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139868
Chromosome:chromosome 10
Location:2905160
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g440050 CSP41A,Csp41a,SNE12,RAP41 (1 of 1) PTHR10366//PTHR10366:SF278 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // CHLOROPLAST STEM-LOOP BINDING PROTEIN OF 41 KDA A, CHLOROPLASTIC; Chloroplast stem-loop-binding protein 41a CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGATCAAGCGCACGGTGCTCCCCGCCAAC
Internal bar code:TTGGAGTCCCAACAACGATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:775
LEAP-Seq percent confirming:75.0565
LEAP-Seq n confirming:1661
LEAP-Seq n nonconfirming:552
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TACAAGGCAAACGTGAGTGC
Suggested primer 2:ATTGTACGACGTAGGCCCTG