| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139868 |
| Chromosome: | chromosome 11 |
| Location: | 1601257 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467762 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAAAAAGGGTTCCGGGTTACCGTTATT |
| Internal bar code: | AGGGTGCGTTGATCTAGTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.1713 |
| LEAP-Seq n confirming: | 359 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGATTGACAAGAGCACA |
| Suggested primer 2: | CGGTATCGTAGCTGGTGAGG |