| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139874 |
| Chromosome: | chromosome 17 |
| Location: | 3984547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728800 | IDH1 | (1 of 1) PTHR11835:SF42 - ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL; Isocitrate dehydrogenase, NAD-dependent | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGAGGGCGGTCGCGGCGGAAGGCGTG |
| Internal bar code: | GACAACGGCACTTCTGTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 99.2429 |
| LEAP-Seq n confirming: | 1704 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCAAGCTTCCCACTTG |
| Suggested primer 2: | CGGTGTGTTTGATGGAGTTG |