Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.139889 |
Chromosome: | chromosome_11 |
Location: | 2071916 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g468850 | FAP152 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCAGCCGCCGCATGCGCTCCTCAGCCAGC |
Internal bar code: | GGGGTGCGAACGGGAGGACTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 944 |
LEAP-Seq percent confirming: | 99.7426 |
LEAP-Seq n confirming: | 6199 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGTAGCGGTGATGTGCTC |
Suggested primer 2: | GTGCATCAACATGAACCCTG |