| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139924 |
| Chromosome: | chromosome 12 |
| Location: | 8099875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551550 | (1 of 1) PF00098//PF02689//PF05970//PF13604 - Zinc knuckle (zf-CCHC) // Helicase (Herpes_Helicase) // PIF1-like helicase (PIF1) // AAA domain (AAA_30) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTGGTATCTGCAATCCCCCCCCCCCT |
| Internal bar code: | TCGCGGATTTTGGTCTGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.6332 |
| LEAP-Seq n confirming: | 5432 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGAGCGAGGAGGAGACC |
| Suggested primer 2: | CCTATGGTGCTGGACGAGTT |