| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139932 |
| Chromosome: | chromosome 2 |
| Location: | 7119121 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCAGGGACCGGCTGCACCCGCGGCCT |
| Internal bar code: | ACCAGTTCGTAAGCCCGGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.6418 |
| LEAP-Seq n confirming: | 47008 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 179 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTCCCGTTCTCACCAGC |
| Suggested primer 2: | TGCGCAGTTGAACTATGGAG |