| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139954 |
| Chromosome: | chromosome 1 |
| Location: | 4681779 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032200 | FAL3 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCCCCGGAAAATACCGGTAATGGTCA |
| Internal bar code: | CCGCCGAGTTAAAGGACGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 70.7205 |
| LEAP-Seq n confirming: | 12299 |
| LEAP-Seq n nonconfirming: | 5092 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGGCAGTGAGCAGGATG |
| Suggested primer 2: | ACGTGTGGCTCATTACCTCC |